Figure 4

Amino acid sequence alignment of several DS domains. (A) Schematic representation of the domain organization of LamA. (B) Sequence alignment, based on the ClustalV method, of the F5/8C module of LamA (Psp; GenBank ABJ15796) with that of Paenibacillus fukuinensis chitosanase-glucanase (Pfu; GenBank BAB64835), Cellvibrio japonicus cbp32B (Cja; GenBank ACE83872), Saccharophagus degradans β-1,3-glucanase (Sde; GenBank ABD82184), retinoschisin (Ret; NP_000321), the C2 domain of human coagulation factor V (Fa5; GenBank AAB59401), the C2 domain of human coagulation factor VIII (Fa8; GenBank AAA52484), and the C2 domain of lactadherin of Bos taurus (Lac; NP_788783). Sequence similarities between the DS domain of Psp and Pfu, Cja, Sde, Ret, Fa5, Fa8, and Lac are 39, 36.9, 44.2, 13.0, 13.0, 14.5, and 16.8%, respectively. Conserved residues are shown in bold, while residues mutated in this study are marked with black dots. Sequences that constitute protruding loops in Fa5, Fa8, and Lac are underlined according to PDB code 1CZS, 1D7P, and 3BN6, respectively. Please note these Proteins can be searched and accessed via http://www.ncbi.nlm.nih.gov/sites/entrez?db=Protein&itool=toolbar