Table 7 Growth and metabolic parameters of E. coli BW25113 (control) Δacs and Δpta mutant strains in batch anaerobic cultures in glucose minimal medium.
Growth parameters | BW25113 | Δpta | Δacs |
|---|---|---|---|
Biomass yield (g·g -1 ) | 0.10 ± 0.003 | 0.05 ± 0.003 | 0.09 ± 0.003 |
Growth rate (h -1 ) | 0.48 ± 0.03 | 0.22 ± 0.02 | 0.43 ± 0.02 |
Glucose uptake rate [mmol·(g·h) -1 ] | 25.40 ± 0.96 | 29.16 ± 0.15 | 25.36 ± 1.11 |
Acetate production rate [mmol·(g·h) -1 ] | 19.15 ± 1.65 | 0.79 ± 0.17 | 16.15 ± 2.09 |
Ethanol production rate [mmol·(g·h) -1 ] | 17.13 ± 3.14 | 2.00 ± 0.39 | 15.72 ± 2.13 |
Formate production rate [mmol·(g·h) -1 ] | 37.34 ± 3.14 | 4.27 ± 0.64 | 31.00 ± 3.73 |
Lactate production rate [mmol·(g·h) -1 ] | 0.91 ± 0.05 | 40.51 ± 5.53 | 0.30 ± 0.01 |
Succinate production rate [mmol·(g·h) -1 ] | 2.19 ± 0.46 | 1.31 ± 0.22 | 1.33 ± 0.15 |