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Table 5 Relative gene expression in E. coli BW25113 Δpta strain.

From: An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli

    Glucose aerobic
batch cultures
Acetate aerobic
batch cultures
  gene pathway Early exponential
phase
Late exponential
phase
Early exponential
phase
Late exponential
phase
rpoD RNA polymerase sigma 70 subunit Transcriptional regulators 0.05 ± 0.02 -0.15 ± 0.04 -0.46 ± 0.23 -0.36 ± 0.10
rpoS RNA polymerase sigma 38 subunit   -0.18 ± 0.22 -0.48 ± 0.05 0.36 ± 0.17 -0.18 ± 0.32
ihfA Integration host factor, α subunit   0.31 ± 0.19 -0.46 ± 0.3 -0.15 ± 0.06 -0.59 ± 0.08
crp cAMP repression protein   0.04 ± 0.15 0.31 ± 0.11 -0.22 ± 0.25 -0.05 ± 0.04
pdhR Pyruvate dehydrogenase complex regulator   -0.70 ± 0.15 -1.11 ± 0.15 n.d. n.d.
fruR cAMP independent protein   -0.19 ± 0.01 -0.20 ± 0.16 -0.59 ± 0.06 -0.05 ± 0.04
zwf Glucose 6-phosphate dehydrogenase Pentose phosphate pathway -0.04 ± 0.02 -0.05 ± 0.03 -0.39 ± 0.06 -0.24 ± 0.04
ptsG Enzyme IIglc, PTS subunit Glucose transport 0.21 ± 0.15 -0.20 ± 0.16 n.d. n.d.
pykF Pyruvate kinase F Glycolysis -0.12 ± 0.08 0.26 ± 0.11 -0.85 ± 0.22 -0.88 ± 0.24
pykA Pyruvate kinase A   -0.25 ± 0.16 0.67 ± 0.11 -0.83 ± 0.10 -0.13 ± 0.06
aceE Pyruvate dehydrogenase   -0.07 ± 0.12 0.00 ± 0.05 -1.04 ± 0.18 -0.16 ± 0.07
ldhA NAD dependent
D-Lactate dehydrogenase
Fermentation pathways -0.02 ± 0.11 0.73 ± 0.18 n.d. n.d.
dld NAD independent
Lactate dehydrogenase
  0.31 ± 0.26 -0.66 ± 0.05 n.d. n.d.
lldD NAD independent
L-lactate dehydrogenase
  -0.09 ± 0.12 -0.41 ± 0.03 n.d. n.d.
adhE Alcohol dehydrogenase   -0.76 ± 0.04 0.21 ± 0.04 n.d. n.d.
pflA Pyruvate formate-lyase
activating enzyme
  -0.09 ± 0.19 1.10 ± 0.10 n.d. n.d.
acs Acetyl-CoA synthethase Acetate metabolism 0.37 ± 0.10 -0.71 ± 0.01 -1.28 ± 0.39 -1.36 ± 0.77
poxB Pyruvate oxidase   -0.94 ± 0.30 -0.40 ± 0.05 -0.82 ± 0.10 -1.00 ± 0.11
actP Acetate Permease   n.d. n.d. -0.28 ± 0.10 -1.13 ± 0.03
ackA Acetate Kinase   n.d. n.d. -0.10 ± 0.01 -0.44 ± 0.00
icdA Isocitrate dehydrogenase TCA cycle 0.27 ± 0.04 -0.30 ± 0.16 -0.82 ± 0.17 0.31 ± 0.47
sucA 2-ketoglutarate dehydrogenase subunit   -0.12 ± 0.19 0.05 ± 0.03 -0.79 ± 0.17 -0.57 ± 0.36
sdhC succinate dehydrogenase membrane protein   -0.14 ± 0.11 -0.77 ± 0.18 0.43 ± 0.58 -0.13 ± 0.47
mdh Malate dehydrogenase   0.15 ± 0.03 -0.28 ± 0.08 -0.87 ± 0.08 -0.52 ± 0.17
aceA Isocitrate lyase Glyoxylate shunt 0.27 ± 0.06 -1.18 ± 0.09 -0.35 ± 0.25 -0.54 ± 0.23
aceB Malate synthase   0.01 ± 0.11 -0.85 ± 0.04 -0.83 ± 0.15 -0.31 ± 0.31
maeB Malate dehydrogenase
(NADP+-requiring)
Glucogenogenesis/anaplerosis 0.25 ± 0.09 -0.26 ± 0.01 -0.39 ± 0.27 0.27 ± 0.34
sfcA/maeA Malate dehydrogenase
(NAD+-requiring)
  0.26 ± 0.04 -0.18 ± 0.03 -0.05 ± 0.23 -0.45 ± 0.28
pck Phosphoenolpyruvate carboxykinase   0.32 ± 0.07 1.04 ± 0.03 -0.57 ± 0.19 0.36 ± 0.13
ppc Phosphoenolpyruvate carboxylase   -0.07 ± 0.08 0.68 ± 0.01 0.21 ± 0.25 0.29 ± 0.03
pps Phosphoenolpyruvate synthase   1.27 ± 0.07 0.64 ± 0.00 -0.39 ± 0.05 -0.07 ± 0.37
  1. Logarithmic ratios were determined using the ΔΔCt method. The E. coli BW25113 (wild type) strain was used as control under each growth condition. Experimental conditions are expressed in Materials and Methods section. (n.d. - not determined).