Figure 2
From: Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli
(A). Comparison of the production of soluble ST6 in Origami™2 cells in the presence of different over-expressed molecular chaperones by Western blotting. (B). Comparison of the production of ST6 (expected molecular weight (MW) ~ 65 KDa) and MBP-ST6 (expected MW ~ 104 KDa) in different fractions of Origami™2 cells by Western blotting. All lanes show similar ST6 band intensities because of signal saturation. Without fusion to MBP, a band corresponding to ST6 could not be detected at the tested short exposure times. Lanes contain equal volumes of bacterial culture. Proteins were probed with an anti-polyHis antibody.