Figure 2From: Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiaeVerification of eYACs in flavonoid producing strains. Separation of eYACs and yeast chromosomes by PFGE (left). Numbers indicate different clones, and M is the Lambda DNA size marker (BioRad) indicating upward increments of approx. 50 kb. Settings were optimized for separation in the 50 – 600 kb area. eYACs are visible in the lower part of the gel after ethidium bromide staining. Confirmation of the eYACs by Southern DNA hybridization (right).Back to article page