Figure 7
Total GFP yields over a 48 h induction period in 1 L fed-batch cultures. The effect of the following induction regimes on cultures grown under standard production conditions (30°C, pH 6.0, 30% DO) and 40 g L-1 glycerol prior to induction are shown: 100% methanol (grey squares); 40% methanol (crosses); 20% methanol (diagonal crosses) and a mixed 60% methanol, 20% sorbitol regime (black diamonds).