Figure 6

Effects of transhydrogenase activity on maximum plasmid-producing flux distributions. For all distributions, α = 250 in Equation (5). Left column:r _{transhydrogenase} limited to 40% of NADPH biomass demand as per Equation (10). Right column:r _{transhydrogenase} constrained to zero; these distributions were copied from Fig. 2 and Fig. 5 to facilitate comparisons. In Equations (8) and (9), r _acetate and r _Pyk were: unconstrained (top row), set to wild-type JM101 values (middle row), or set to Pyk-deficient PB25 values (bottom row). For simplicity, the network in Fig. 1 has been condensed and fluxes (mmol/g/h) have been expressed as % of glucose uptake, except r _Bla (expressed as % of total protein) and r _plasmid (expressed as yield in mg/g). The production of lactate and succinate have also been omitted, as these fluxes were nil for all distributions.