Figure 5
From: Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint
Maximum plasmid-producing flux distributions for the JM101 and PB25 models. For both distributions, α = 250 in Equation (5), and r transhydrogenase was constrained to zero. Equations (8) and (9) were employed to constrain r acetate and r Pyk to experimental values for JM101 (wild-type) and PB25 (pykF pykA). Top: JM101. Bottom: PB25. For simplicity, the network in Fig. 1 has been condensed and fluxes (mmol/g/h) have been expressed as % of glucose uptake, except r Bla (expressed as % of total protein) and r plasmid (expressed as yield in mg/g). The production of lactate and succinate have also been omitted, as these fluxes were nil for all distributions.