Figure 4From: Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain RandTime course of protease activity and pH of the medium. Culture media were incubated at 37°C with shaking at 150 rpm for 48 h. Samples were taken at 4 h intervals to determine the protease activity and the pH level.Back to article page