Table 2 Oligonucleotide primers used for DNA inserts in plasmid construction.
Insert | Primer sequence | Restriction sites/feature |
|---|---|---|
GFP insert 1 | GGG GAATTC ATGAGTAAAGGAGAAGAACTTT | EcoRI |
| Â | GGG TCTAGA GGCGGCGGC TTTGTAGAGCTCATCCATGCC | XbaI, AAA |
GFP insert 2 | GCG CCGCGG TG ATGAGTAAAGGAGAAGAACTTT | SacII |
| Â | GGG TCTAGA CC TGGACCACCACC TTTGTATAGTTCATCCATGCCA | XbaI, GGGP |
LARD0 insert | GCG ATCGAT A (CCAGGT) × 5 TCGTCCCTCGGCGTGCATG | ClaI, PGPGPGPGPG |
| Â | GCG GGTACC TCAACTGATCAGCACACCCT | KpnI |
LARD-1 insert | AC TCTAGA ATTGAAGGACGA TCCATCGCCAACCTGTCG | XbaI, F.Xa |
| Â | GGG AAGCTT ATGAACCGCCGATAATCCGT | HindIII |
TliA insert 1 | AC TCTAGA ATTGAAGGACGA ATGGGTGTATTTGACTACAAGA | XbaI, F.Xa |
| Â | GGG AAGCTT ATGAACCGCCGATAATCCGT | HindIII |
TliA insert 2 | GGG GGTACC GGAGGA ATGGTGATTTGACTACAAGA | KpnI, GG |
| Â | GGG AAGCTT TCAACTGATCAGCACACCCT | HindIII |
TliA insert 3 | GGG ATCGAT GGA ATGGGTGTATTTGACTACAAGA | ClaI, G |
| Â | GGG GGATCC TCCTCC ACTGATCAGCACACCCTCG | BamHI |
EGF insert | GGG GAATTC ATGAATAGTGACTCTGAATGTCC | EcoRI |
| Â | GGG TCTAGA GCGCAGTTCCCACCACTTC | XbaI |
GFP-LARD0 insert | GCG GGTACC CCAGGTGGT ATGACCATGATTACGCCAAG | KpnI PGPG |
| Â | TAATACGACTCACTATAGGG | (KpnI) |
GFP-TliA insert | CG GGTACC CCAGGT ATGAGTAAAGGAGAAGAACTTT | KpnI, PG |
GFP-LARD1 insert | ATCTTCTCTCATCCGCCAAA | (HindIII) |