Microbial Cell Factories

Table 1 Characteristics of plasmids used in this study.

From: Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD)

Plasmids	Properties/inserts	Vector/Source
pGFPuv	Cycle 3 variant of GFP	pUC/Clontech
pGFP-TliA	GFP insert 1, TliA insert1	pKK223-3 [48]
pGFP-SXold	intermediate to pGFP-LARD0	pBluescript-SK(+)
pGFP-LARD0	GFP insert 1, LARD0 insert	pBluescript-SK(+)
pGFP-LARD1	GFP insert 2, LARD1 insert	pKK223-3
pEGF-TliA	EGF insert, TliA insert1	pKK223-3
pEGF-LARD1	EGF insert, LARD1 insert	pKK223-3
pCTP-TliA	TliA insert 2	pCTP [21]
pTliA-CTP	TliA insert 3	pCTP
pCTP-GFP-TliA*	GFP-TliA insert	pCTP
pCTP-GFP-LARD0	GFP-LARD0 insert	pCTP
pCTP-GFP-LARD1	GFP-LARD1 insert	pCTP
pABC-ACYC	tliDEF from P. fluorescens	[12]
pEcPrtDEF-184	prtDEF from E. chrysanthemi	pRUW4 [49]

The name of plasmids indicates the order of different genes. For example, pCTP-GFP-TliA* denotes a fusion protein consisting of CTP, GFP, and TliA in order.

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ISSN: 1475-2859

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