Figure 5

Immunoblot analysis of recombinant proteins in culture supernatants. E. coli harboring two plasmids was grown for 48 h and 16 μl of the culture supernatant was subjected to SDS-PAGE and analyzed by immunoblot analysis using anti-TliC antibody. E, E. chrysanthemi PrtDEF (E. coli harboring pEcPrtDEF-184 grown at 37°C); B, P. fluorescens TliDEF (E. coli harboring pABC-ACYC grown at 25°C); N, no ABC transporter (pACYC-184). 1, pTliA; 2, pGFP-TliA; 3, pGFP-LARD1; 4, pGFP-LARD0; 5, pEGF-TliA; 6, pEGF-LARD1.