Skip to main content

Table 2 BMS1 up-regulation can be used as a marker for selecting high-yielding strains

From: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Strain Copies BMS1 mRNA/cell Fps1 yield relative to wild-type
wild-type 0.1 (0.1) 100 (27.0)
spt8Δ 0.2 (0.0) 104 (28.0)
med6Δ 0.2 (0.1) 55.6 (15.0)
yTHC SRB6 0.8 (0.3)** 310 (12.0)**
  1. Analysis of mRNA was performed as described in the legend to Table 1, and of Fps1 yield as described in the Methods section. Standard deviation in parentheses was calculated for the samples (n = 4). A double asterisk denotes significance at the P < 0.01 level (paired 2-tailed T-test).SPT8 encodes a subunit of the SAGA complex, required for inhibition at some promoters. MED6 and SRB6 encode subunits of the mediator complex.