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Table 1 Fps1 yield correlates with BMS1 transcript number in the host cell

From: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

  Copies mRNA/cell
Strain TPI1 FPS1 BMS1
wild-type 53.6 (5.0) 60.1 (18.2) 0.1 (0.0)
gcn5Δ 15.4 (3.4)** 61.5 (3.0) 0.7 (0.2)**
spt3Δ 12.5 (1.8)** 64.9 (26.0) 0.6 (0.1)**
srb5Δ 38.2 (12.5)* 87.4 (1.5) 0.7 (0.2)**
  1. Analysis of mRNA from shake-flask cultures was performed using real time quantitative PCR. The data were normalized using the reference genes ACT1 and PDA1 and the signal was scaled to mRNA copies/cell according to a SAGE study [32], in which copies of mRNA/cell of the reference genes had previously been determined. Standard deviation in parentheses was calculated for the samples (n = 4). A single asterisk denotes significance at the P < 0.05 level (paired 2-tailed T-test), and a double asterisk at P < 0.01 level.