Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Table 1 Fps1 yield correlates with BMS1 transcript number in the host cell

Analysis of mRNA from shake-flask cultures was performed using real time quantitative PCR. The data were normalized using the reference genes ACT1 and PDA1 and the signal was scaled to mRNA copies/cell according to a SAGE study [32], in which copies of mRNA/cell of the reference genes had previously been determined. Standard deviation in parentheses was calculated for the samples (n = 4). A single asterisk denotes significance at the P < 0.05 level (paired 2-tailed T-test), and a double asterisk at P < 0.01 level.

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