Table 1 Site-specific forward (pyk-F) and reverse (pyk-R) primer sequences used for verification of deletion of the pyk gene encoding pyruvate kinase in C. glutamicum by PCR.
From: Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum
Primer direction | Primer sequence |
|---|---|
Forward | pyk-F: 5'-GATCC TCGAG CTCTA CTGAG CTGGT TTAT -3' |
Reverse | pyk-R: 5'-GATCG GATCC CGGTC AACAT AGAGC TCA -3' |