Target | Primer sequence | Position and sequence accession number | Length | Tm |
---|---|---|---|---|
plasmid | 5'-CGGTTGCTGGCGCCTAT-3' | 703–721 pET 3a, Novagen | 17 bp | 83°C |
5'-ACCATACCCACGCCGAAA-3' | 780–797 pET 3a, Novagen | 18 bp | ||
chromosome | 5'-GCGAGCGATCCAGAAGATCT-3' | 4044875 – 4044894 E. coli K12, Accession number U00096 | 20 bp | 75°C |
5'-GGGTAAAGGATGCCACAGACA-3' | 4044941–4044961 E. coli K12, Accession number U00096 | 21 bp |