Figure 4From: Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processesVariation of PCN and hG-CSF accumulation level in fermentation. Escherichia coli BL21 (DE3) strain transformed with pET3a plasmid with gene for hG-CSF was used. Fermentation was preformed in GYSP medium without ampicillin (a) and with ampicillin (b) at 25°C for 25 h.Back to article page