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Table 4 Effect of varied activity ratio for Ct XR and Cb FDH during whole cell-catalyzed reduction of 100 mM ethyl 4-cyanobenzoylformate by a suitable mixture of E. coli XR_FDH and E. coli FDH.

From: Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli

substrate conc. [mM] XR : FDH additive1,2 yield [mM]3/ee [%]4
100 1 : 2 polymyxin B 18/86.1 R
100 1 : 6 polymyxin B 36/74.6 R
100 1 : 20 polymyxin B 62/71.3 R
100 1 : 2 NAD+, polymyxin B 23/66.8 R
100 1 : 6 NAD+, polymyxin B 36/64.7 R
100 1 : 20 NAD+, polymyxin B 64/71.2 R
500 1 : 2 polymyxin B 25/≥ 99.9 R
500 1 : 6 polymyxin B 39/84.6 R
500 1 : 20 polymyxin B 88/73.7 R
500 1 : 2 NAD+, polymyxin B 29/≥ 99.9 R
500 1 : 6 NAD+, polymyxin B 42/85.1 R
500 1 : 20 NAD+, polymyxin B 90/75.5 R
  1. The activity of Ct XR was kept constant at 1.4 U/mL throughout.
  2. 1 [NAD+] = 490 μM, 2 [polymyxin B sulphate] = 144 μM;3Standard errors ≤ 15%. 4Detection limit of ethyl 4-cyanomandelate is below 0.01 mM.