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Table 3 Effect of additives and co-solvents on yield and enantiomeric excess of whole-cell bioreduction of ethyl 4-cyanobenzoylformate catalyzed by E. coli XR_FDH.

From: Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli

substrate conc. [mM] org. co-solvent1 additive2,3 yield [mM]4/ee [%]5
100    82/97.2 R
100   NAD+ 85/97.1 R
100   polymyxin B 75/97.0 R
100   NAD+, polymyxin B 77/97.2 R
100 ethyl acetate   1/> 99.9 R
100 ethyl acetate NAD+ 9/> 99.9 R
100 hexane   3/> 99.9 R
100 hexane NAD+ 7/> 99.9 R
500    134/> 99.9 R
500   NAD+ 136/> 99.9 R
500   polymyxin B 125/> 99.9 R
500   NAD+, polymyxin B 126/> 99.9 R
500 butyl acetate   9/> 99.9 R
500 butyl acetate NAD+ 35/> 99.9 R
500 BMIMPF6   74/> 99.9 R
500 BMIMPF6 NAD+ 108/> 99.9 R
  1. Conditions: 40 gCDW/L; 8.5 h reaction time. 1Co-solvents were added in 1:1 volume ratio. 2 [NAD+] = 490 μM. 3 [polymyxin B sulphate] = 144 μM. 4Standard errors ≤ 15%. 5Detection limit of ethyl 4-cyanomandelate is below 0.01 mM.
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Microbial Cell Factories

ISSN: 1475-2859

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