Figure 7
From: Engineering inclusion bodies for non denaturing extraction of functional proteins
SDS-PAGE analysis of proteins extracted from IBs with 0.2% N-lauroyl sarcosine. Inclusion bodies were isolated from E. coli cultivated at 25°C. 1 – BSA (Bovine serum albumin) standard 0.2 mg; 2 – BSA standard 0.88 mg; 3 – BSA standard 1.5 mg; 4 – total cell proteins; 5 – soluble (cytoplasmic) proteins; 6 – IBs proteins; 7 – IBs proteins extracted with 0.2% NLS; 8 – in 0.2% NLS insoluble IBs proteins; 9 – LMW standard.