Table 2 Assay program at 'eMicroLISA'
From: Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC)
Sequence | Task | Conditions (Temperature [°C]/Time [s]) |
|---|---|---|
1 | Wash flow | RT/20 |
2 | Rehydration and temperature change | 50/250 |
3 | Sample transfer | 50/7 |
4* | Hybridization | 50/20 |
5* | Sample renewal | 50/12 |
6 | Wash flow | 50/140 |
7 | Enzyme conjugate transfer | 50/6 |
8 | Incubation | 50/250 |
9 | Wash flow | 50/70 |
10 | Temperature change | 55/25 |
11 | Substrate transfer | 55/65 |
12 | Stop flow and readout | 55/35 |
13 | Wash flow | RT/30 |