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Table 1 Biomass yield, cell dry weight and side product concentration for E. coli W3110 during the different cultivation types on glucose based mineral salt medium.

From: High cell density media for Escherichia coli are generally designed for aerobic cultivations – consequences for large-scale bioprocesses and shake flask cultures

Type of cultivation Addition of trace elements YX/S1 (g g-1) CDW (g L-1) Acetate (mM) Lactate (mM) Formate (mM)
   Batch2 Fed-batch3 Batch Fed-batch Batch Fed-batch Batch Fed-batch Batch Fed-batch
Control + 0.49 (0.01)4 n.d. 19.3 (1.1) n.d. 43.8 (10.1) n.d. 4.5 (6.3) n.d. 0.5 (0.7) n.d.
Control - 0.48 (0.01) 0.55 18.9 (0.4) 36.4 30.8 (8.4) 29.7 3.8 (3.4) 11.6 1.8 (2.0) 5.0
STR-PFR +   0.50   37.8   0.1   2.2   0.0
STR-PFR -   0.45   36.4   4.5   0.0   14.8
Oxygen downshift +   0.23   20.2   139.7   435.8   0.0
Oxygen downshift -   0.38   24.7   173.2   60.6   16.9
  1. 1 Yield coefficients were calculated for a time period of 4 hours starting with the feeding start. Here YX/S includes also biomass formed from acetate which is consumed during this phase.
  2. 2 Batch samples were taken at the end of the exponential growth phase.
  3. 3 Fed-batch sample was taken 4 h after the feed start.
  4. 4 Numbers in brackets indicate standard deviations between different cultivations.