Table 2 Oligonucleotide primers used in this study.
From: Nisin inducible production of listeriolysin O in Lactococcus lactis NZ9000
Primer code number | Primer name | Primer sequence (5'-3') (restriction enzyme site or overhang)a |
|---|---|---|
1 | pQE30 forward primer | GAAGGATCC GATGCATCTGCATTCAATAAAG (Bam HI site) |
2 | pQE30 reverse primer | ACGCCTGCAG TTCGATTGGATTATCTACTTTATTA (Pst I site) |
3 | PnisA forward primer | CCAAGATCT AGTCTTATAACTATACTG (Bgl II site) |
4 | PnisA reverse primer (hly overhang) | GGTGATGTCCCAT TTTGAGTGCCTCCTTATAATTTATTTTG (hly overhang) |
5 | hly forward primer (PnisA overhang) | AGGCACTCAAA ATGGGACATCACCATCACCATCACGGA (PnisA overhang) |
6 | hly reverse primer | AGTCGGTACC TTATTCGATTGGATTATCTAC (Kpn I site) |