Table 3 General guidelines for good lab praxis.
Clone only in expression vectors that allow cleavage of the tag after purification. Advantage: any side effect due to the tag can be evaluated | Â |
|---|---|
Cleave the tag before using the target protein in interaction experiments. Advantage: unspecific interactions can be limited | Â |
Prepare mono-use aliquots of the purified protein. Advantage: reproducibility of the experiment is improved | Â |
Perform an experiment to evaluate protein stability after incubation on ice or at room temperature at different times; stability for freeze/thaw, if applicable. Advantage: experiment design is optimized | Â |