Table 1 Material information sheet. The MI concerning the material preparation and characterization is reported
Construct information | Accession number; partial sequence?; mutations?; fusions to tags?; rec. Abs: format (Fab, scFv, VHH) |
|---|---|
Vector information | Vector type and map; cloning sites; resistance; linkers; protease cleavage sites |
Hybridoma clone | Identification; tests in ELISA, IP, WB, IHC |
Chromatographic steps | IMAC (column1, buffer 1); Desalting (column 2, buffer 2); ..... |
Tag cleavage | Buffer; cleavage conditions |
Protein storage conditions | Buffer; temperature; aliquots; |
Re-folding | Buffer; strategy, glycosylation |
Functionality | Enzymatic assay; IP; ELISA; IHC; SPR; ITC; Oxidation status (SH/S-S), Appearance, Stability |
Purification parameters | Yields; Specific activity; KD; Aggregation index; multimerisation, specific activity |
Purity | host cells proteins, nucleic acids, lipids, carbohydrates, process added chemicals |