Figure 5

AmyΔCt-BlaM chimera secretion in P. haloplanktis TAC125 wild type and gspE mutant. Panel A: Western blotting analyses of extra-cellular media of P. haloplanktis TAC125(pFCamy ΔCt-blaM) recombinant cells. Samples were collected during the early (24 h) (lane 1), and middle (32 h) (lane 2) exponential phase. Immunodetection was performed by chemioluminescence after probing proteins with anti-α-amylase (anti-Ph α-Amy) and anti-β-lactamase (anti-Ec Bla) polyclonal antisera. Panel B: Western blotting analyses of extra-cellular media of P. haloplanktis TAC125 gspE mutant-(pFCamy ΔCt-blaM) recombinant cells. Samples were collected during the early (24 h) (lane 3), and middle (32 h) (lane 4) exponential phase. The immunodetections were performed by using anti-α-amylase (anti-Ph α-Amy) and anti-β-lactamase (anti-Ec Bla) polyclonal antisera.