Table 2 Hydroxylase and epoxidase activities in crude protein extract of recombinant S. cerevisiae strains, YpAC3-15(4) and YH6H 2-2 overexpressing the B. candida h6h gene.
Crude extract | Hydroxylase activity | Epoxidase activity |
|---|---|---|
Specific Activity (nKat mg-1) | Specific Activity (nKat mg-1) | |
Untagged H6H | 2.60 ± 0.19 | 0.24 ± 0.02 |
Tagged H6H | 0.89 ± 0.06 | ND* |