Table 1 Strains and plasmids used in this study.
Relevant genotype | Xylose pathway | Reference | |
|---|---|---|---|
Plasmids | |||
YEplacHXT | bla HXT7p-PGK1t URA3 | - | [29] |
YEpHXT-XIp | bla HXT7p-XI (Piromyces)-PGK1t URA3 | XI | This study |
S. cerevisiae | |||
TMB 3044 | CEN.PK 2-1C, Δgre3, his3::PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t, | - | [29] |
TMB 3057 | TMB 3044, pY7 (ADH1p-XYL1-ADH2t, PGK1p-XYL2-PGK1t URA3) | XR-XDH-XK | [29] |
TMB 3066 | TMB 3044, YEpHXT-XIp (HXT7p-XI-PGK1t URA3) | XI-XK | This study |
TMB 3400 | HIS3::(ADH1p-XYL1-ADH2t, PGK1p-XYL2-PGK1t, PGK1p-XKS1-PGK1t) | XR-XDH-XK | [42] |