Protein

Activity^{a} (%)

Molecular mass^{b}


pdα_{1}PI (standard)

100

50,300

deglypdα_{1}PI

n.a.^{c}

44,210

rα_{1}PI/E. coli

35^{d}

45,000^{e}

rα_{1}PI/A. niger

76^{f,g}

50,100

 ^{a}The calculations were based on comparison with pdtα_{x}tPI as a standard (100%) and using normalized equal concentrations of rα_{1}PI samples as determined by ELISA; ^{b}molecular weight is shown as a an average value measured for 2 samples; ^{c} not available; pdα_{1}PI was enzymatically deglycosylated under denaturing conditions; ^{d} as measured for Histagged rα_{1}PI within 1.5 h after elution from the TALON beads; ^{e} as estimated by amino acid sequence for α_{1}PI without glycans; ^{f} shown for A. niger supernatant after Amicon 10 K filtration procedure; ^{g} the standard deviation of our potency assay is ± 15%.