Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

Table 5 Recovery of adenine nucleotides after spiking extracts with a known amount of ATP.

Amounts of ATP, ADP and AMP that were determined in the cell extract before and after the addition of 6.1 nmoles of ATP. The relative standard deviation for the ATP quantifications was less than 5%. The sample was taken from a batch culture that was growing on CDM with 333 mM of glucose without quenching and the cell pellet was extracted with cold methanol. The energy charge value calculated from the energetic metabolites is 0.28 ± 0.01.

ISSN: 1475-2859