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Table 2 ATP leakage (%) with four different quenching procedures.

From: Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

  Batch culture Chemostat culture (D = 0.06 h-1)
Supernatants MeOH MeOH/HEPES MeOH/NaCl MeOH/HEPES MeOH/NaCl MeOH/AC
Quenched 12.0 6.4 9.5 4.3 15.0 8.1
1 st washed 14.7 1.2 4.8 0.5 5.4 0.8
Total 26.7 7.6 14.3 4.8 20.4 8.9
  1. Percentage of ATP that was measured in the supernatant of L. plantarum cells after quenching and washing with different quenching solutions that contained either 60% methanol, 60% methanol and 70 mM of HEPES (pH 5.5), 60% MeOH and 0.85% NaCl or 60% methanol and 0.85% ammonium carbonate (pH 5.5). Samples were taken from batch or continuous cultures, grown on CDM with 333 mM and 100 mM of glucose respectively. The percentage was calculated by dividing the amount of ATP in the supernatant by the sum of the amounts of ATP in the supernatants and cell extract. The average leakage of ATP in the supernatant of non-quenched batch culture samples was 5%.