Figure 8
From: Hitting bacteria at the heart of the central dogma: sequence-specific inhibition
Synthesis of single-stranded DNAzymes. The ssDNA was synthesized by a reverse transcriptase from moloney murine leukemia virus (MoMuLV), the gene of which was placed downstream from a hybrid PLtetO-1 promoter in the vector. This was followed by coding sequences for a reverse transcription termination structure, a DNAzyme, a primer binding site (PBS), and a terminator. The MoMuLV reverse transcriptase was synthesized from the resulting transcript encoding reverse transcriptase, reverse transcription terminator, DNAzyme, and PBS. Endogeneous tRNA(Val) then hybridized to the PBS and primed the reverse transcription of the single-stranded DNAzyme, which was stopped by the reverse transcription terminator.