Figure 1
From: Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans
Immunodetection of SK in extracellular fractions. Proteins from culture supernatants were precipitation with a mixture of chloroform and methanol (1:3, v/v). In each lane, proteins according to 100 μl spent culture medium were loaded. Lane 1, S. lividans [pOW15] 40 h; lane 2, 60 ng SK standard; lane 3, S. lividans [pOVsiSK] 30 h; lane 4, S. lividans [pOVsiSK] 40 h; lane 5, S. lividans [pOXlnCSK] 30 h; lane 6, S. lividans [pOXlnCSK] 40 h.