Figure 7
From: Development of expression vectors for Escherichia coli based on the pCR2 replicon
Expression of h-IFNγ in E.coli BL21 (DE3) containing pCR2-IFNγ. a) Total cell lysates run on 15% SDS-PAGE at different time points post induction. Lane 1: Pre induction sample. Lane 2: 4 hrs sample. Lane 3: 6 hrs sample. Lane 4: Protein molecular weight marker. (b) Western blot profiles showing expression of pCR2-IFNγ at the above time points. (c) Comparative expression of h-IFNγ in E.coli BL21 (DE3) cells vis-a-vis expression in E.coli DH5α heat shock system. Total cell lysate run on 15% SDS-PAGE 4 hrs post induction Lane 1: IPTG induced E.coli BL21 (DE3) containing pCR2-IFNγ. Lane 2: Temperature induced E.coli DH5α containing pCR2-IFNγ and pGP1-2.