Figure 2
From: Evidence for glycosylation on a DNA-binding protein of Salmonella enterica
Dps binds to jacalin, via CRD, but not to other galactose-binding lectins. Isolectin B4 of Griffonia simplicifolia (GSIB4), euphorbin from Euphorbia milli, jacalin and KM+ from Artocarpus integrifolia, and concanavalin-A were biotinylated and their interactions with Dps in solid phase were assessed (A). The negative control was performed in the absence of lectins. The binding of biotinylated jacalin to Dps in solid phase was assessed in the presence of sugars at a concentration of 0.1 M (B). The alpha-anomer of methyl-galactose (Jac-αgal) inhibited the jacalin/Dps binding drastically, whereas the beta anomer (Jac-βgal) did not. A solution of D-galactose (Jac-αβgal) and D-mannose (Jac-man) partially inhibited this interaction. PBS was the control in the absence of sugars and lectins.