Figure 1

Production of CzrB-6his in E. coli BL21 (DE3) cells. Lane 1. Molecular weight marker; lane 2. E. coli control; lanes 3,4. whole cell extracts after CzrB production at 25°C and 37°C; lanes 5,6. membrane fractions (concentrated 3.5 × relative to whole extracts) after CzrB production at 25°C and 37°C.