Figure 1
From: Genomic and proteomics approaches for vaccine development in Pasteurella multocida
Different P . multocida strains pm1578 mutant. Shown are (A) PCR amplification of P. multocida vaccine target ORFs for Gateway cloning. (B) SDS-PAGE and Coomassie Blue staining of crude lysates expressing P. multocida X-73 recombinant proteins in E. coli DH5α. All cultures were induced with arabinose at mid-log phase (C) Isolation of target proteins from inclusion bodies. Coomassie Blue staining of 14 recombinant proteins solubilised in 8 M Urea. (D) Proteins in lysates were separated by electrophoresis and the immunoblot was probed with mouse antiserum raised against PM1578.