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Table 2 Differences in carbon dioxide secretion rate and in requirements regarding ammonium, sulphates and oxygen when the metabolism is optimized for recombinant protein production as opposed to biomass production.

From: Application of a genome-scale metabolic model to the inference of nutritional requirements and metabolic bottlenecks during recombinant protein production in Escherichia coli

  Flux Variation (a) Flux Variation (b)
NH4 requirements 16% 9%
O2 requirements -27% -15%
SO4 requirements 31% 17%
CO2 secretion rate -23% -13%