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Figure 1 | Microbial Cell Factories

Figure 1

From: Set up and optimization of a fermentation protocol for the production of a human antibody fragment (Fab') express in E. coli. Pre-pilot and cGMP pilot scale studies

Figure 1

a) Effect of DO-Stat strategy on: (♦) biomass level (OD600), (■) residual substrate concentration and (▲) Fab' accumulation at 10 liters scale fermentation (HPLC analysis); b typical curve of (–) DO, (–) pH, (---) OD600 and (---) Inductor (FEED) during DO-Stat fermentation; c representative curves of (–) conductivity and (–) capacitance in fermentation broth and Oxygen and CO2 in exhausted air; d Western blot of samples taken throughout the induction phase

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