Table 1 hGH periplasmic secretion level activating at different temperature and utilizing hGH-secreting W3110 strains, with or without the repressor gene (cIts).
temperature | phGH-DsbA-λPL+ pRK-248 cIts (μg/mL/A600 ± SD) | phGH-DsbA-λPL(μg/mL/A600 ± SD) |
|---|---|---|
30°C | 0.01 | 1.0 ± 0.14 (n = 2) |
35°C | 0.02 | - |
37°C | 0,06 | - |
42°C | 1,31 ± 0.38 (n = 4) | 1.61 ± 0.11 (n = 3) |