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Table 1 Protein expression yield (μg/mL/OD) and final optical densities (OD600) of different strains with pL promoter.

From: Point mutation of serine 179 in the human Prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities

 

E. colistrain

Protein yield (μg/mL/OD)

Final OD600

PRL

W3110

1.3 ± 0.2

4.0 ± 0.3

 

BL21

1.9 ± 0.4

1.3 ± 0.2

 

BL21 codon plus

1.4 ± 0.3

1.0 ± 0.2

S179D PRL

W3110

0.34 ± 0.03

3.8 ± 0.6

 

BL21

0.35 ± 0.5

1.3 ± 0.5

 

BL21 codon plus

0.40 ± 0.3

1.2 ± 0.1

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Microbial Cell Factories

ISSN: 1475-2859

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