Figure 2From: Heterologous overexpression of a halophilic α-amylase(a) Effect of NaCl concentration on the folding efficiency of α-amylase inclusion bodies dissolved in 8 M urea. Refolding buffer: 20 mM Tris-HCl pH 7.4 with salt. (b) Effect of calcium concentration on the folding efficiency of α-amylase inclusion bodies dissolved in 8 M urea. Refolding buffer: 20 mM Tris-HCl pH 7.4, 3 M NaCl and calcium.Back to article page