vector | α-amylase (UI/ml) | α-amylase | alkaline phosphatase | secretion yielda |
|---|
| | p | em | em(%) | em(%) | |
pFFamyΔ Ct-dsbA | 0.12 ± 0.01 | 3.58 ± 0.03 | 97 | 4 |
93
|
pFFamyΔ Ct-trpC | 0.07 ± 0.02 | 3.65 ± 0.03 | 98 | 5 |
93
|
pFFamyΔ Ct-blaM | 1.23 ± 0.03 | 6.21 ± 0.03 | 83 | 1 |
82
|
- Data are average results of three independent experiments. The volume of periplasmic fraction was made the same as corresponding extra-cellular medium to allow a comparison. UI, international units; p, periplasmic extract; em, extra-cellular medium fraction; em(%), percentage of the activity in extra-cellular medium fraction of the total activity (periplasmic plus extra-cellular medium fractions).
- a The difference between the em (%) of amylase activity and em (%) of alkaline phosphatase activity.
