Table 3 Chemostat studies of methylotrophic yeasts [90, 102, 107, 114, 118, 119].
From: Regulation of methanol utilisation pathway genes in yeasts
| Â | Â | AOX | CAT | FLD | FDH |
|---|---|---|---|---|---|
Methanol-limited chemostat | C. b. | Decreasing with increasing dilution rate | Relatively constant over the D range tested | Increasing with increasing dilution rate | Increasing with increasing dilution rate |
| Â | H. p. | Decreasing with increasing dilution rate | Increasing with increasing dilution rate | Increasing with increasing dilution rate | Increasing with increasing dilution rate |
Glucose-limited chemostat | C. b. | Low level of derepression at D's < 0.2 h-1 | Increasing with increasing dilution rate | High level of derepression at D's < 0.4 h-1 | Low level of derepression at D's < 0.1 h-1 |
| Â | H. p. | High level of derepression at D's < 0.4 h-1 | Increasing with increasing dilution rate | High level of derepression at D's < 0.4 h-1 | Low level of derepression at D's < 0.2 h-1 |
Glucose/methanol fed chemostat | C. b. | Decreasing with increasing D | Increasing with increasing D up to 0.3 h-1 | Activity peak at D ~0.12 h-1 | Almost constant at D < 0.3 h-1 |
| Â | H. p. | Decreasing with increasing D | Increasing with increasing D up to 0.3 h-1 | Increasing with increasing D up to 0.3 h-1 | Increasing with increasing D up to 0.3 h-1 |
Sorbitol-limited chemostat | H. p. | Decreasing with increasing dilution rate | Decreasing with increasing dilution rate | Decreasing with increasing dilution rate | Decreasing with increasing dilution rate |
Sorbitol/methanol fed chemostat | H. p. | Decreasing with increasing dilution rate | Increasing with increasing dilution rate | Increasing with increasing D above 0.3 h-1 | Activity peak at D = 0.37 h-1 |
Glycerol/methanol fed chemostat | H. p. | High specific enzyme activity at D = 0.1 h-1 | High specific enzyme activity at D = 0.1 h-1 | High specific enzyme activity at D = 0.1 h-1 | High specific enzyme activity at D = 0.1 h-1 |
Ethanol/methanol fed chemostat | H. p. | No activity found at D = 0.1 h-1 | No activity found at D = 0.1 h-1 | No activity found at D = 0.1 h-1 | No activity found at D = 0.1 h-1 |