Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism

Table 3 Specific enzyme activities in E. coli strains BL21, BL21/pOri1 and BL21/pOri2 cultured in the chemostat system at different growth rates.

^a Abbreviations: HEK, Hexokinase; GPI, Glucose-6-phosphate isomerase; PFK, 6-phosphofructosekinase; FBP, Fructose-1,6-bisphosphatase; FBA, Fructose-bisphosphate aldolase; GPD, Glyceraldehyde-3-phosphate dehydrogenase; TPI, Triose phosphate isomerase; PPC, Phosphoenolpyruvate carboxylase; PK, Pyruvate kinase; GPDH, Glucose-6-phosphate dehydrogenase; PGL, 6-Phosphogluconolactonase; PGD, Phosphogluconate dehydrogenase; RPE, Ribulose-phosphate 3-epimerase; RPI, Ribose-5-phosphate isomerase; TK, Transketolase; TA, Transaldolase; CS, Citrate synthase; ICD, NADP⁺-specific isocitrate dehydrogenase; ICL, Isocitrate lyase; AH, Aconitate hydratase; OGD, Oxoglutarate dehydrogenase; SD, Succinic semialdehyde dehydrogenase; FH, Fumarate hydratase; MD, Malate dehydrogenase; MAC, Malic enzyme; PCK, Phosphoenolpyruvate carboxykinase.
^b The following growth rates were determined when bacteria were cultured in the batch cultivation: BL21, 0.46 h^-1; BL21/pOri1, 0.39 h^-1; BL21/pOri2, 0.29 h^-1.
^c The experiments were performed in triplicate and the results are shown as the average of three measurements with the standard deviation less than 5%.

ISSN: 1475-2859