Table 1 List of the constructs used in the experiments. The features of the vectors are indicated along with the expressed insert.
Vector | Insert | Promoter | Inducer | Origin |
|---|---|---|---|---|
pBADM | GST, ClipA5 | ara BAD | arabinose | pUC |
pTrcHis2 | GST, ClipA5 | trc | IPTG | pUC |
pKK223 | GST, ClipA5 | tac | IPTG | pBR322 |
pQE30 | GST, ClipA5 | T5/lac | IPTG | colE1 |
pETM | GST, ClipA5 | T7/lac | IPTG | pBR322 |
pGAT | GST | T7 | IPTG | pUC |
pZS24MCS | GST, ClipA5 | Plac/ara-1 | arabinose | pSC101 |