Figure 4From: Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approachData for model calibration. Cultures performed in shake flask and induced at 3 h and 5 h for 1 h from 30°C to 42°C, as indicated by the gray boxes. Data were used for the determination of the model kinetic parameters showed in Table 1. Symbols refer to experimental data and lines to model simulation (model fitting).Back to article page