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Table 1 Time evolution of the secondary structure in both β-galactosidase thermal aggregates and VP1LAC inclusion bodies as measured by FTIR peak ratios.

From: The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells

Proteina

Time (h)

Ratio 1627/1652b

Ratio 1692/1652b

β-galactosidase

1

1.31

0.42

 

3

1.53

0.40

 

5

1.63

0.52

VP1LAC

1

1.36

0.54

 

3

2.14

0.83

 

5

2.21

0.96

  1. aData are from Figure 3
  2. bPeaks at 1627 cm-1 and 1692 cm-1 can be attributed to extended intermolecular β-sheet while that at 1652 to disordered structure and/or α-helix.
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Microbial Cell Factories

ISSN: 1475-2859

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