Table 1 A comparison of the miniature bioreactor systems (MBRs) that have been reported to be capable of parallel operation illustrating key technical and performance specifications.
From: Miniature bioreactors: current practices and future opportunities
Device/Reference | Based on | Volume (ml) | Agitation/Aeration | pH, DOT and OD instrumentation | KLa (h-1) | Method | Multiplexing |
|---|---|---|---|---|---|---|---|
Fedbatch-Pro, http://www.dasgip.de | Shake flask | 50 – 500 | Orbital shaker; Surface | pH via sterilisable probe | N/R | N/A | 16 |
MicroReactor, http://www.applikon-bio.com | MTP + microfabrication | 3–5 | Orbital shaker (up to 800 rpm); Gas sparging | pH and DOT via optical probes | N/R | N/A | 24 |
SimCell, http://www.bioprocessors.com | Microfluidic chip | 0.3 – 0.7 | Rotation of MBR chips; Surface via membrane | pH, DOT and OD at-line via cell-reading station | Up to 500 | CFD estimation | 1500 |
MBR array [59] | MTP + microfabrication | 0.25 | Orbital shaker (175 rpm); electrochemical O2 generation | pH (ISFET sensor) and OD optically | Unknown | N/A | 8 |
Polymer-MBR [41, 56] | Microfabrication | 0.15 | Magnetic stirrer bar (200–800 rpm), Surface via membrane | pH, DOT and OD via optical probes | 20 – 75 | Dynamic gassing-out | 8 |
Stirrer-Pro flask, http://www.dasgip.de | STR | 200 – 275 | Magnetic stirrer bar (10 – 1000 rpm); Sparger | pH and DOT via sterilisable probes | N/R | N/A | 16 |
Xplorer, http://www.bioxplore.net | STR | Up to 100 | Single turbine impeller (100 – 2000 rpm); Sparger | pH, DOT and OD probes | 400 | Dynamic gassing-out | 16 |
Cellstation, http://www.fluorometrix.com | STR | Up to 35 | Dual paddle impeller (10 – 1000 rpm); Sparger | pH, DOT and OD via optical probes | N/R | N/A | 12 |
MSBR [27, 40] | STR | 18 | Triple turbine impeller (up to 7000 rpm); Sparger | pH and DOT via optical probes | Up to 480 | Dynamic gassing-out | N/R |
Bioreactor block [39, 44] | STR | 8 – 12 | Gas-inducing single impeller (up to 4000 rpm) | DOT optically; pH and OD via plate reader | 700 – 1600 | Dynamic gassing-out | 48 |
Parallel BCR [52, 53] | Bubble column | 200 | Gas-sparging | pH and DOT probes | Up to 540 | Dynamic gassing-out | 16 |
MBCR [49, 51] | Bubble column | 2 | Gas-sparging | pH and DOT via optical probes | Up to 220 | Dynamic gassing-out | 48 |