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Table 1 A comparison of the miniature bioreactor systems (MBRs) that have been reported to be capable of parallel operation illustrating key technical and performance specifications.

From: Miniature bioreactors: current practices and future opportunities

Device/Reference Based on Volume (ml) Agitation/Aeration pH, DOT and OD instrumentation KLa (h-1) Method Multiplexing
Fedbatch-Pro, http://www.dasgip.de Shake flask 50 – 500 Orbital shaker; Surface pH via sterilisable probe N/R N/A 16
MicroReactor, http://www.applikon-bio.com MTP + microfabrication 3–5 Orbital shaker (up to 800 rpm); Gas sparging pH and DOT via optical probes N/R N/A 24
SimCell, http://www.bioprocessors.com Microfluidic chip 0.3 – 0.7 Rotation of MBR chips; Surface via membrane pH, DOT and OD at-line via cell-reading station Up to 500 CFD estimation 1500
MBR array [59] MTP + microfabrication 0.25 Orbital shaker (175 rpm); electrochemical O2 generation pH (ISFET sensor) and OD optically Unknown N/A 8
Polymer-MBR [41, 56] Microfabrication 0.15 Magnetic stirrer bar (200–800 rpm), Surface via membrane pH, DOT and OD via optical probes 20 – 75 Dynamic gassing-out 8
Stirrer-Pro flask, http://www.dasgip.de STR 200 – 275 Magnetic stirrer bar (10 – 1000 rpm); Sparger pH and DOT via sterilisable probes N/R N/A 16
Xplorer, http://www.bioxplore.net STR Up to 100 Single turbine impeller (100 – 2000 rpm); Sparger pH, DOT and OD probes 400 Dynamic gassing-out 16
Cellstation, http://www.fluorometrix.com STR Up to 35 Dual paddle impeller (10 – 1000 rpm); Sparger pH, DOT and OD via optical probes N/R N/A 12
MSBR [27, 40] STR 18 Triple turbine impeller (up to 7000 rpm); Sparger pH and DOT via optical probes Up to 480 Dynamic gassing-out N/R
Bioreactor block [39, 44] STR 8 – 12 Gas-inducing single impeller (up to 4000 rpm) DOT optically; pH and OD via plate reader 700 – 1600 Dynamic gassing-out 48
Parallel BCR [52, 53] Bubble column 200 Gas-sparging pH and DOT probes Up to 540 Dynamic gassing-out 16
MBCR [49, 51] Bubble column 2 Gas-sparging pH and DOT via optical probes Up to 220 Dynamic gassing-out 48
  1. Nomenclature: SF = shake flask, MTP = microtitre plate, MSBR = miniature stirred bioreactor, STR = stirred tank reactor, MBCR = miniature bubble column reactor, DOT = dissolved oxygen, OD = optical density, ISFET = ion-selective field effect transistor, N/A = not applicable, N/R = not reported.