Protein | Promoter | Â | Substrate | Total protein (mg/L)1 (U/mL)2 | Productivity (mg/L h)1 (U/L h)2 | Operational mode | Bioreactor | Reference |
---|---|---|---|---|---|---|---|---|
Insect esterase | GAP | Intracellular | Glucose | 71 | -- | Batch | Shake flasks 50 mL | [94] |
 |  | Extracellular |  | 801 | -- |  |  |  |
 | AOX1 | Extracellular | Methanol | 401 | -- |  |  |  |
Fructose exo-levanase | GAP | Extracellular | Glycerol | 26.62 | 6822 | Fed-batch | Bioreactor 7.5 L | [20] |
 | AOX1 | Extracellular | Methanol | 21.12 | 2202 | Fed-batch |  |  |
Human trysinogen | GAP | Extracellular | Glycerol | -- | -- | Fed-batch | Bioreactor 2 L | [21] |
 | AOX1 | Extracellular | Methanol | -- | -- | Fed-batch |  |  |
Vitellogenin | GAP | Intracellular | Glucose | 122 | -- | Fed-batch | Bioreactor 2 L | [98] |
hGM-CSF | GAP | Extracellular | Glucose | 901 | 1.21 | Batch | Shake flasks 50 mL | [93] |
 | GAP+AOX1 | Extracellular | Methanol | 1801 | 2.41 | Batch |  |  |
Aqualysin I | GAP | Extracellular | Glucose | 10001 | Â | Batch | Shake flasks | [100] |
h-Chitinase | GAP | Extracellular | Glucose | 4501 | 2.81 | Fed-batch | Bioreactor 3 L | [84] |
 |  |  |  | -- | 151 | Continuous |  |  |
 | AOX | Extracellular | Methanol | 3501 | 2.91 | Fed-batch |  |  |
HBsAg | GAP | Extracellular | Glucose | -- | -- | Fed-batch | Shake flasks 500 mL | [96] |
 | GAP multicopy | Extracellular |  | -- | -- | Fed-batch |  |  |
 |  |  |  | -- | -- | Cyclic batch |  |  |
 | AOX1 | Extracellular | Methanol | -- | -- | Fed-batch |  |  |
Cellobiohydrolase | GAP | Extracellular | Glycerol | -- | -- | Fed-batch | Bioreactor 7.5 L | [92] |
 | AOX1 | Extracellular | Methanol | -- | -- | Fed-batch |  |  |