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Table 6 PGAP promoter cultures comparison

From: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Protein Promoter   Substrate Total protein
(mg/L)1
(U/mL)2
Productivity
(mg/L h)1
(U/L h)2
Operational mode Bioreactor Reference
Insect esterase GAP Intracellular Glucose 71 -- Batch Shake flasks 50 mL [94]
   Extracellular   801 --    
  AOX1 Extracellular Methanol 401 --    
Fructose exo-levanase GAP Extracellular Glycerol 26.62 6822 Fed-batch Bioreactor 7.5 L [20]
  AOX1 Extracellular Methanol 21.12 2202 Fed-batch   
Human trysinogen GAP Extracellular Glycerol -- -- Fed-batch Bioreactor 2 L [21]
  AOX1 Extracellular Methanol -- -- Fed-batch   
Vitellogenin GAP Intracellular Glucose 122 -- Fed-batch Bioreactor 2 L [98]
hGM-CSF GAP Extracellular Glucose 901 1.21 Batch Shake flasks 50 mL [93]
  GAP+AOX1 Extracellular Methanol 1801 2.41 Batch   
Aqualysin I GAP Extracellular Glucose 10001   Batch Shake flasks [100]
h-Chitinase GAP Extracellular Glucose 4501 2.81 Fed-batch Bioreactor 3 L [84]
     -- 151 Continuous   
  AOX Extracellular Methanol 3501 2.91 Fed-batch   
HBsAg GAP Extracellular Glucose -- -- Fed-batch Shake flasks 500 mL [96]
  GAP multicopy Extracellular   -- -- Fed-batch   
     -- -- Cyclic batch   
  AOX1 Extracellular Methanol -- -- Fed-batch   
Cellobiohydrolase GAP Extracellular Glycerol -- -- Fed-batch Bioreactor 7.5 L [92]
  AOX1 Extracellular Methanol -- -- Fed-batch